Soybean [Glycine max (L.) Merill] is a crop with nutritional and economic value and a soil improvement crop. Soybean belongs to a group of crops with poor resistance to adverse abiotic factors. Therefore, research to improve the ability of soybean plants to withstand abiotic stresses is essential in the context of climate change. This study aims to analyze the molecular evolution of the AP2 gene family, the DREB gene subfamily and the AP2 domain to select candidate genes for improving soybean resistance. Using BioEdit, BLAST and MEGA11 software to search, compare data and analyze phylogeny, the results show that the phylogenetic tree reflects the diversity and evolution of genes in the AP2 family and the DREB subfamily and AP2 domain in soybean. At the same time, 18 DREB genes were identified located on 17 soybean chromosomes. Among the DREB genes, the functions of some genes have yet to be thoroughly studied and need to be verified experimentally.