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Correlations between abnormalities of morphological details and DNA fragmentation in human sperm
Home Research Publications Correlations between abnormalities of morphological details and DNA fragmentation in human sperm

Correlations between abnormalities of morphological details and DNA fragmentation in human sperm

Tuyet Nguyen Thi Hiep, Hong Nhan Thi Dang, Thai Thanh Thi Nguyen, Trung Van Nguyen, Thuan Cong Dang, Quoc Huy Vu Nguyen, Minh Tam Le, Mar-2022, In: Clinical and Experimental Reproductive Medicine (CERM), Original Article, p. 1-9

Overview

  • Tuyet Nguyen Thi Hiep
  • Hong Nhan Thi Dang
  • Thai Thanh Thi Nguyen
  • Trung Van Nguyen
  • Thuan Cong Dang
  • Quoc Huy Vu Nguyen
  • Minh Tam Le

Abstract:

Objective

As the associations of sperm DNA fragmentation with morphology have not been examined in detail, this study aimed to investigate the relationship between abnormalities of morphological details and DNA integrity in human sperm.

Methods

In this cross-sectional study, men from infertile couples were enrolled at Hue Center for Reproductive Endocrinology and Infertility, Vietnam. Conventional semen parameters, including morphological details, were analyzed following the World Health Organization 2010 criteria. Sperm DNA fragmentation was evaluated using a sperm chromatin dispersion assay. The relationships and correlations between semen parameters, sperm morphology, and the type of halosperm and the DNA fragmentation index (DFI) were analyzed.

Results

Among 130 men in infertile couples, statistically significant differences were not found in the sperm halo type between the normal and abnormal sperm morphology groups. The percentage of round-head spermatozoa was higher in the DFI >15% group (16.98%±12.50%) than in the DFI ≤15% group (13.13%±8.82%), higher values for amorphous heads were found in the DFI >15% group, and lower values for tapered heads were observed in the DFI ≤15% group; however, these differences were not statistically significant. Small-halo sperm and the DFI were positively correlated with round-head sperm (r=0.243, p=0.005 and r=0.197, p=0.025, respectively).

Conclusion

The rate of general sperm morphological abnormalities in semen analysis was not related to sperm DNA integrity. However, round sperm heads were closely associated with sperm DNA fragmentation.

Keyword(s): Anatomy and histology; DNA fragmentation; Infertility; Male; Spermatozoa

Pages (from-to) 1-9
Journal Clinical and Experimental Reproductive Medicine (CERM)
Volume Original Article
Publication status Published - Mar-2022
ISBN 1226-2951