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Physiological intracytoplasmic sperm injection does not improve the quality of embryos: A cross-sectional investigation on sibling oocytes
Home Research Publications Physiological intracytoplasmic sperm injection does not improve the quality of embryos: A cross-sectional investigation on sibling oocytes

Physiological intracytoplasmic sperm injection does not improve the quality of embryos: A cross-sectional investigation on sibling oocytes

Le Minh Tâm, Tuyet Nguyen Thi Hiep, Nguyễn Văn Trung, Nguyễn Thị Thái Thanh, Đặng Thị Hồng Nhạn, Đặng Công Thuận, Nguyễn Vũ Quốc Huy, May-2023, In: Clinical and Experimental Reproductive Medicine (CERM), 50, 2, p. 123-131

Overview

  • Le Minh Tâm
  • Tuyet Nguyen Thi Hiep
  • Nguyễn Văn Trung
  • Nguyễn Thị Thái Thanh
  • Đặng Thị Hồng Nhạn
  • Đặng Công Thuận
  • Nguyễn Vũ Quốc Huy

Abstract:

Objective: This study aimed to compare the efficacy of physiological intracytoplasmic sperm injection (PICSI) and intracytoplasmic sperm injection (ICSI) in terms of the fertilization rate and embryo quality using sibling oocyte cycles. Methods: This prospective, cross-sectional study collected data from 76 couples who underwent their first cycle at the Hue Center for Reproductive Endocrinology and Infertility, Vietnam, between May 2019 and November 2021. The inclusion criteria were cycles with at least eight oocytes and a sperm concentration of 5×106 /mL. Sperm parameters, sperm DNA fragmentation (SDF), fertilization, and the quality of cleavage-stage embryos on day 2 and blastocysts on day 5 were examined. Results: From 76 ICSI cycles, 1,196 metaphase II (MII) oocytes were retrieved, half of which were randomly allocated to either the PICSI (n=592) or ICSI (n=604) treatment group. The results showed no significant difference between the two groups in terms of fertilization (72.80% vs. 75.33%, p=0.32), day 2 cleavage rate (95.13% vs. 96.04%, p=0.51), blastulation rate (52.68% vs. 57.89%), and high-quality blastocyst rate (26.10% vs. 31.13%, p=0.13). However, in cases where SDF was low, 59 cycles consisting of 913 MII oocytes produced a considerably higher blastulation rate with PICSI than with ICSI (50.49% vs. 35.65%, p=0.00). There were no significant differences between the pregnancy outcomes of the PICSI and ICSI embryo groups following embryo transfer. Conclusion: Using variable sperm quality provided no benefit for PICSI versus ICSI in terms of embryo outcomes. When SDF is low, PICSI appears to be able to produce more blastocysts. 

Keyword(s): DNA fragmentation; Embryonic structures; Fertilization in vitro; Hyaluronic acid; Sperm injections, intracytoplasmic

Pages (from-to) 123-131
Journal Clinical and Experimental Reproductive Medicine (CERM)
Volume 50
Issue number 2
Publication status Published - May-2023
ISBN 1226-2951