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Simultaneous determination of vitamin B6 and magnesium using capillary electrophoresis coupled with contactless conductivity detection: Method development, validation, and application to pharmaceutical and nutraceutical samples
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Simultaneous determination of vitamin B6 and magnesium using capillary electrophoresis coupled with contactless conductivity detection: Method development, validation, and application to pharmaceutical and nutraceutical samples

Huy Nguyen Quang, Sep-2022, In: Journal of Chromatography B

Overview

Abstract:

Vitamins and minerals are usually incorporated in pharmaceutical and nutraceutical products, but a simple, rapid, and inexpensive analytical method for their simultaneous determination is still lacking. In this study, we developed a quantification method for pyridoxine (vitamin B6) and magnesium (Mg) by using purpose-made

capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D) instrument. Main analytical conditions include: fused silica capillary (total length 55 cm, effective length 40 cm, inner

diameter 50 μm); background electrolyte consisted of 10 mM L-arginine/acetic acid (pH 5) with 20% acetonitrile; separation voltage + 20 kV; hydrodynamic injection (siphoning at 20 cm in 25 s). Detection limits of vitamin B6 and Mg were 1 and 0.1 mg/L, respectively. Good linearity (R2 > 0.999) was observed for vitamin B6

and Mg calibration curves over concentration ranges of 3–100 and 0.3–200 mg/L, respectively. The method was

applied to analyze vitamin B6 and Mg in several pharmaceutical and nutraceutical samples. The analytical results obtained by our method were in good agreement with reference methods (i.e., HPLC for vitamin B6 and ICP-OES for Mg). High-efficient and low-cost CE-C4D method can accordingly serve as a promising tool for concurrent analysis of inorganic and organic species in pharmaceutical and nutraceutical analysis.

Keyword(s): CE-C4D Magnesium Vitamin B6 Pharmaceutical Nutraceutical

Journal Journal of Chromatography B
Publication status Published - Sep-2022