STUDY OF CONDITIONS OF SEPARATION β-LACTAM ANTIBIOTICS BY REVERSED PHASE CHROMATOGRAPHY
A T. N. T., , 2011, In: Vietnam Journal of Chemistry, 49/2, 2, p. 908-912
Overview
Abstract:
In the paper, conditions of separation 5
β-lactam antibiotics, consist of ampicillin – AMP, penicillin G – PENG,
oxacillin – OXA, cloxacillin – CLO, cephalexin – CEP by RP-HPLC are described.
The column is Supelcosil ODS (C18), 250mm×4,6mm, 5µm particles. The mobile
phase of RP-HPLC is mixture of ACN/MeOH/buffer of 10/20/70 (v/v), pH 3,5. The limted of detection of 5 β-lactam antibiotics is 0,01 - 0,1 μg/ml.
The method determined AMP and CEP in urine of recovery of 86,33% đến 90,62%.
Pages (from-to) | 908-912 |
Journal | Vietnam Journal of Chemistry |
Volume | 49/2 |
Issue number | 2 |
Publication status | Published - 2011 |
ISBN | 0866-7144 |