In  continuation  of  developing  new  cytotoxic  compounds,  six  azacrownophanes  containing  both  2,4,6-triarylpyri-dine and 2,6-bis(phenoxymethyl)pyridine moieties were synthesized successfully by one-step domino-condensation of podand 2,6-bis[(2-acetophenyl)oxymethyl]pyridine, arylaldehydes and ammonium acetate according to the condi-tions of Hantzsch reactions. The compounds 5a-c were selected for cytotoxicity evaluations against human cell lines (HepG2, Lu1, RD, FL and MCF-7). Compound 5b showing the highest cytotoxicity is interesting for the development of promising anticancer drugs.In continuation of developing new cytotoxic compounds, six azacrownophanes containing both 2,4,6-triarylpyridine and 2,6-bis(phenoxymethyl)pyridine moieties were synthesized successfully by one-step domino-condensation of podand 2,6-bis[(2-acetophenyl)oxymethyl]pyridine, arylaldehydes and ammonium acetate according to the conditions of Hantzsch reactions. The compounds 5a-c were selected for cytotoxicity evaluations against human cell lines (HepG2, Lu1, RD, FL and MCF-7). Compound 5b showing the highest cytotoxicity is interesting for the development of promising anticancer drugs.