Objective: To evaluate the correlation between the sperm fragmentation DNA and the outcome of the intracytoplasmic sperm injection (ICSI) technique. Materials and methods: The descriptive cross-sectional study on 175 infertile couples by ICSI method at the Center for Reproductive Endocrinology and Infertility, Hue University Hospital. Evaluation of sperm DNA fragmentation by using sperm chromatin dispersion technique. After 16-18 hours of injection, the fertilized oocytes were determined by the presence of two pronuclei. Embryos were evaluated according to Istanbul consensus on the day 2 and 5. Results: The rate of fertilization in group DFI ≥ 30% was difference statistically lower than in DFI group <30% (80.27 ± 17.47% vs. 74.48 ± 17.32%, p = 0.046). There was no difference statistically between the two groups DFI for the quality of embryos on day 2. The rate of usable embryos on day 5 in group DFI ≥ 30% was lower than that of DFI group <30%, the difference was not significant statistics (79.97 ± 25.39 compared to 85.93 ± 18.43%, p = 0.081). Sperm DNA fragmentation had a correlation with the fertilization rate (r = -0.185, p = 0.014), linear Regression equation: y = - 0.187x + 83.55. Conclusion:  Sperm DNA fragmentation has a negative correlation with post-ICSI fertilization rates. Sperm DNA fragmentation assay has a valuation in predict the results of infertile treatment.